December 6th, 2019

NVPW meeting, Hotel de Nieuwe Wereld, Marijkeweg 5, Wageningen

Listen to and discuss with Iris “IFC concepts for DH protocol developement and optimization”

Modern plant breeding uses doubled haploid plants (DHs) in genetic studies for trait identification and integration into their breeding lines as well as to generate homozygous parental lines for F1 hybrid seed production. Unfortunately, the development of a DH-protocol can be time and cost intensive due to the numerous factors affecting microspore viability. Impedance flow cytometry (IFC) is an established method for pollen analysis in plant breeding and seed production. As IFC discriminates quickly and reliable between dead and viable cells it has also a great potential to identify critical success factors for each step of a DH-protocol supporting its development and optimization.


September 19-20th, 2019.

5th Amphacademy at Amphasys, Technopark Luzern, CH 6039 Root-D4, Switzerland

Iris will talk about “IFC concepts for DH protocol developement and optimization”

Doubled haploid plants (DH) have an important role in modern plant breeding and F1 hybrid seed production. The generation of DHs is influenced by various factors and not always successful and efficient. IFC with its easy, quick, and reliable discrimination between dead and viable cell has the potential being an elegant screening tool to identify critical success factors at an early stage throughout a DH-protocol; examples will be shown during this presentation.

Methods belonging to this presentation will be published in 2020 in Springer MIMB on Doubled Haploid Technology edited by Jose-Maria Segui-Simarro.




September 14-15th, 2018

Amphacademy at Amphasys, Technopark Luzern, CH 6039 Root-D4, Switzerland

The 4th Amphacademy, all about pollen and cell quality. As one of the first users in plants, Iris will be around for discussions.


August 19-24th, 2018

IABP meeting in Dublin, Ireland

On Tuesday August 21st, session 3C on Abiotic and Biotic Stresses (Liffey Meeting Room 2)

Iris will talk about:

Pollen analysis made easy


Success of doubled haploid production, interspecific crosses, breeding strategies, and seed production depends highly on pollen quality which is affected by genetic and environmental factors. The recently developed microchip-based impedance flow cytometry (IFC) detects changes in the electrical properties of cells and enables a reliable, non-destructive analysis of pollen. Within a single measurement it is now possible to discriminate between dead, viable, and pollen with germination capacity. This technique allows to identify plants with distorted pollen development, to screen for abiotic stress tolerance, to improve and develop pollen storage protocols, and to optimize F1 hybrid seed production. These and other applications will be presented.


June 12-15th, 2018.

Flowertrials in the Netherlands 

Iris will be visiting the Aalsmeer region on june 12 and 13th.


January 23rd, 2018

IPM Essen, Germany

Contact Acepo for an appointment and a chat about pollination problems


January 15th, 2018

Speciality of Acepo: Pollen viability analysis in orchids


September 15-16th, 2017

Amphacademy, at Amphasys, Technopark Luzern, CH 6039 Root D4, Switzerland

Iris will talk about:

Viability Analysis of Orchid Pollen


June 9th, 2017

NVPW spring symposium. Start 9.30 at Hotel De Nieuwe Wereld, Marijkeweg 5, 6709 PE Wageningen.

Iris will talk about:

A new tool in pollen analysis


Pollen quality defined by viability and germination capacity is essential for fruit and seed production in commercial and breeding industries. The classical methods estimating pollen quality are based on various staining methods or classical in vitro germination protocols that are not easily applied across species, time consuming, and not always related to each other. This presentation will show how pollen quality is analyzed by an impedance flow cytometer using the natural cellular reaction to an electric field. This method does not require staining, is reliable, species independent, and standardisable. It can be used to detect pollen developmental defects, discriminate between dead and viable cell, and even to predict pollen germination